Human protein: IPI00640320 * 50122 Da * CDC25C Isoform 2 of M-phase inducer phosphatase 3 - Liebel-Lab @ KIT
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>CDC25C Isoform 2 of M-phase inducer phosphatase 3 - Length: 443 AS 
MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSG
EITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEI
SDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITIT
QMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQ
EELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQ
SYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP
Linkouts to other data resources: BioCompare * Entrez * PolyMeta * GeneCard * imaGenes * GenomeBrowser * Google Scholar * Mitocheck
AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page
Diseaselink to OMIM


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page
ACTIVATE: SMART analysis
NameBeginEndE-value
low complexity623-
Pfam:M-inducer_phosp932421.20e-49
RHOD2813952.00e-17

These features and domains are not shown in the diagram, either because their scores are less significant than the required threshold, or because they overlap with some other source of annotation:

NameBeginEndE-valueReason
Pfam:Rhodanese2843921.30e-34overlap


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page

AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page

AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page

AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page


AceView - BLAST - CDD - ensEMBL - EntrezGene - GoPubMed - H-Inv - IHOP - Localisation - MINT - OMIM - ProteinWiki - SMART - UNIPROT - STRING - crosslinks top of page
General information
Entry name MPIP3_HUMAN
Accession number P30307, Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1
Integrated 01-APR-1993, UniProtKB/Swiss-Prot.
Sequence update 17-OCT-2006, sequence version 2
Annotation update 25-NOV-2008, entry version 92
UniSave P30307, Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1
UniRef100 UniRef100_P30307, UniRef100_P30307-4, UniRef100_P30307-3, UniRef100_P30307-2
UniParc UPI000002AE3E, UPI000002AE3F, UPI000013E3D2, UPI000020C6C6
Description and origin of the Protein
Description Recommended
Full=M-phase inducer phosphatase 3;
EC=3.1.3.48;
Synonym
Full=Dual specificity phosphatase Cdc25C;
Gene name(s) CDC25C
Organism source Homo sapiens (Human).
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
NCBI TaxID 9606
References
[1] Sadhu,K., Reed,B.I., Richardson,H., Russell,P.,
Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
(1990) Proc. Natl. Acad. Sci. U.S.A. 87:5139-5143
Position NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND VARIANT CYS-70.
Medline 90311358
PubMed
2195549 Subscription requiredClick to get it from PaperBase@EBI FreeClick to get it from PubMedCentral FreeClick to get it from PubMedCentral FreeClick to get it from UK_PubMedCentral FreeClick to get it from UK_PubMedCentral Free after 6 monthsClick to get it from www.pnas.org Free after 6 monthsClick to get it from www.pnas.org Subscription requiredClick to get it from www.pnas.org CiteXplore
[2] Bureik,M., Rief,N., Drescher,R., Jungbluth,A., Montenarh,M., Wagner,P.,
An additional transcript of the cdc25C gene from A431 cells encodes a functional protein.
(2000) Int. J. Oncol. 17:1251-1258
Position NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
Medline 20532852
PubMed
11078813 CiteXplore
[3] Livingston,R.J., Rieder,M.J., Chung,M.-W., Ritchie,T.K., Olson,A.N., Nguyen,C.P., Nguyen,D.A., Poel,C.L., Robertson,P.D., Schackwitz,W.S., Sherwood,J.K., Sherwood,A.M., Leithauser,B.J., Nickerson,D.A.,
NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
Submitted DEC-2003 to the EMBL GenBank DDBJ databases
Position NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
(2004) Genome Res. 14:2121-2127
Position NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT CYS-70.
Comments TISSUE=Skin;
DOI 10.1101/gr.2596504;
PubMed
15489334 Free after 6 monthsClick to get it from www.genome.org Free after 6 monthsClick to get it from www.genome.org FreeClick to get it from www.genome.org Subscription requiredClick to get it from DOI Subscription requiredClick to get it from PaperBase@EBI FreeClick to get it from PubMedCentral FreeClick to get it from PubMedCentral FreeClick to get it from UK_PubMedCentral FreeClick to get it from UK_PubMedCentral CiteXplore
[5] Wegener,S., Hampe,W., Herrmann,D., Schaller,H.C.,
Alternative splicing in the regulatory region of the human phosphatases CDC25A and CDC25C.
(2000) Eur. J. Cell Biol. 79:810-815
Position NUCLEOTIDE SEQUENCE [MRNA] OF 32-210 (ISOFORMS 2; 4 AND 5), AND VARIANT CYS-70.
Medline 21020092
DOI 10.1078/0171-9335-00115;
PubMed
11139144 Subscription requiredClick to get it from ScienceDirect CiteXplore
[6] Hernandez,S., Bessa,X., Bea,S., Hernandez,L., Nadal,A., Mallofre,C., Muntane,J., Castells,A., Fernandez,P.L., Cardesa,A., Campo,E.,
Differential expression of cdc25 cell-cycle-activating phosphatases in human colorectal carcinoma.
(2001) Lab. Invest. 81:465-473
Position PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5).
Comments TISSUE=Colon carcinoma;
PubMed
11304565 CiteXplore
[7] Lu,R., Niida,H., Nakanishi,M.,
Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function.
(2004) J. Biol. Chem. 279:31164-31170
Position PHOSPHORYLATION AT SER-216.
Comments TISSUE=Testis;
DOI 10.1074/jbc.M404728200;
PubMed
15150265 Open AccessClick to get it from intl.jbc.org Open AccessClick to get it from intl.jbc.org Open AccessClick to get it from intl.jbc.org Subscription requiredClick to get it from DOI Subscription requiredClick to get it from PaperBase@EBI CiteXplore
[8] Goh,W.C., Manel,N., Emerman,M.,
The human immunodeficiency virus Vpr protein binds Cdc25C: implications for G2 arrest.
(2004) Virology 318:337-349
Position INTERACTION WITH HIV-1 VPR, AND MUTAGENESIS OF GLU-352 AND LYS-359.
DOI 10.1016/j.virol.2003.10.007;
PubMed
14972559 Subscription requiredClick to get it from PaperBase@EBI Subscription requiredClick to get it from ScienceDirect CiteXplore
[9] Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J., Gygi,S.P.,
A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
(2006) Nat. Biotechnol. 24:1285-1292
Position PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168, AND MASS SPECTROMETRY.
Comments TISSUE=Epithelium;
DOI 10.1038/nbt1240;
PubMed
16964243 Subscription requiredClick to get it from nature.com Subscription requiredClick to get it from PaperBase@EBI CiteXplore
[10] Dephoure,N., Zhou,C., Villen,J., Beausoleil,S.A., Bakalarski,C.E., Elledge,S.J., Gygi,S.P.,
A quantitative atlas of mitotic phosphorylation.
(2008) Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767
Position PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; THR-48; SER-57; SER-61; SER-64 AND THR-67, AND MASS SPECTROMETRY.
DOI 10.1073/pnas.0805139105;
PubMed
18669648 Free after 6 monthsClick to get it from www.pnas.org Free after 6 monthsClick to get it from www.pnas.org Free after 6 monthsClick to get it from www.pnas.org Subscription requiredClick to get it from www.pnas.org CiteXplore
Comments
FUNCTION Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDC2 and activate its kinase activity.
CATALYTIC ACTIVITY Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.
SUBUNIT Interacts with HIV-1 Vpr, thereby inactivating CDC25C phosphatase activity.
INTERACTION
P06493:CDC2 NbExp=1 IntAct=EBI-974439, EBI-444308
O14757:CHEK1 NbExp=2 IntAct=EBI-974439, EBI-974488
Q9Y250:LZTS1 NbExp=1 IntAct=EBI-974439, EBI-1216080
Q14738:PPP2R5D NbExp=1 IntAct=EBI-974439, EBI-396563
SUBCELLULAR LOCATION Nucleus.
ALTERNATIVE PRODUCTS Event=Alternative splicing; Named isoforms=5;
Name=1; Synonyms=CDC25C1;
IsoId=P30307-1; Sequence=Displayed;
Name=2; Synonyms=CDC25C2;
IsoId=P30307-2; Sequence=VSP_000864;
Name=3; Synonyms=CDC25C3;
IsoId=P30307-5; Sequence=Not described;
Name=4; Synonyms=CDC25C4;
IsoId=P30307-3; Sequence=VSP_000863;
Name=5; Synonyms=CDC25C5, Cdc25Cdm;
IsoId=P30307-4; Sequence=VSP_000863, VSP_000864;
DEVELOPMENTAL STAGE Expressed predominantly in G2 phase.
PTM Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase.
SIMILARITY Belongs to the MPI phosphatase family.
SIMILARITY Contains 1 rhodanese domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms Distributed under the Creative Commons Attribution-NoDerivs License
Database cross-references
EMBL
M34065; AAA35666.1; -; mRNA.
AJ304504; CAC19192.1; -; mRNA.
AY497474; AAR32098.1; -; Genomic_DNA.
BC019089; AAH19089.1; -; mRNA.
AF277723; AAG41885.1; -; mRNA.
AF277725; AAG41887.1; -; mRNA.
AF277726; AAG41888.1; -; mRNA.
AF312681; AAL26835.1; -; mRNA.
PIR
I59168; A38874.
RefSeq
NP_001781.2; -.
NP_073720.1; -.
UniGene
Hs.656; -.
PDB
3BZI; X-ray; 2.10 A; E=126-134.
PDBsum
3BZI; -.
DIP
DIP:24183N; -.
IntAct
P30307; -.
PhosphoSite
P30307; -.
Ensembl
ENSG00000158402; Homo sapiens.
GeneID
995; -.
KEGG
hsa:995; -.
H-InvDB
HIX0005209; -.
HGNC
HGNC:1727; CDC25C.
HPA
CAB003800; -.
MIM
157680; gene.
PharmGKB
PA100; -.
HOGENOM
P30307; -.
HOVERGEN
P30307; -.
Reactome
REACT_152; Cell Cycle, Mitotic.
REACT_1538; Cell Cycle Checkpoints.
NextBio
4178; -.
ArrayExpress
P30307; -.
CleanEx
HS_CDC25C; -.
GermOnline
ENSG00000158402; Homo sapiens.
GO
GO:0005829; C:cytosol; EXP:Reactome.
GO:0005654; C:nucleoplasm; EXP:Reactome.
GO:0004725; F:protein tyrosine phosphatase activity; NAS:UniProtKB.
GO:0050699; F:WW domain binding; IPI:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0008283; P:cell proliferation; TAS:UniProtKB.
GO:0006260; P:DNA replication; EXP:Reactome.
GO:0044419; P:interspecies interaction between organisms; IEA:UniProtKB-KW.
GO:0006470; P:protein amino acid dephosphorylation; IEA:InterPro.
GO:0000079; P:regulation of cyclin-dependent protein kina...; TAS:UniProtKB.
GO:0007088; P:regulation of mitosis; TAS:ProtInc.
GO:0007089; P:traversing start control point of mitotic c...; TAS:ProtInc.
InterPro
IPR000751; MPI_Phosphatase.
IPR001763; Rhodanese-like.
Gene3D
G3DSA:3.40.250.10; Rhodanese-like; 1.
PANTHER
PTHR10828; MPI_Phosphatase; 1.
Pfam
PF06617; M-inducer_phosp; 1.
PF00581; Rhodanese; 1.
PRINTS
PR00716; MPIPHPHTASE.
SMART
SM00450; RHOD; 1.
PROSITE
PS50206; RHODANESE_3; 1.
Protein Existence
1: Evidence at protein level;
Keywords
3D-structure; Alternative splicing; Cell cycle; Cell division; Host-virus interaction; Hydrolase; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Protein phosphatase;
CHAIN: 1 473, M-phase inducer phosphatase 3. /FTId=PRO_0000198647. DOMAIN: 321 428, Rhodanese. REGION: 334 379, HIV-1 Vpr binding site. ACT_SITE: 377 377, By similarity. MOD_RES: 38 38, Phosphoserine. MOD_RES: 48 48, Phosphothreonine. MOD_RES: 57 57, Phosphoserine. MOD_RES: 61 61, Phosphoserine. MOD_RES: 64 64, Phosphoserine. MOD_RES: 67 67, Phosphothreonine. MOD_RES: 168 168, Phosphoserine. MOD_RES: 216 216, Phosphoserine; by CHK1, CHK2 and BRSK1. VAR_SEQ: 66 123, GTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQE VHLAGMNHDQHLMKCSP -> SPGFFRTSGSAFSWD (in isoform 4 and isoform 5). /FTId=VSP_000863. VAR_SEQ: 124 153, Missing (in isoform 2 and isoform 5). /FTId=VSP_000864. VARIANT: 14 14, S -> N (in dbSNP:rs11567959). /FTId=VAR_027922. VARIANT: 70 70, R -> C (in dbSNP:rs3734166). /FTId=VAR_027923. VARIANT: 78 78, S -> N (in dbSNP:rs11567962). /FTId=VAR_027924. VARIANT: 297 297, G -> R (in dbSNP:rs11567997). /FTId=VAR_020146. MUTAGEN: 352 352, E->K: Partial loss of HIV-1 Vpr binding. MUTAGEN: 359 359, K->E: No effect on HIV-1 Vpr binding. STRAND: 127 129, CHAIN: 1 473, M-phase inducer phosphatase 3. /FTId=PRO_0000198647. DOMAIN: 321 428, Rhodanese. REGION: 334 379, HIV-1 Vpr binding site. ACT_SITE: 377 377, By similarity. MOD_RES: 38 38, Phosphoserine. MOD_RES: 48 48, Phosphothreonine. MOD_RES: 57 57, Phosphoserine. MOD_RES: 61 61, Phosphoserine. MOD_RES: 64 64, Phosphoserine. MOD_RES: 67 67, Phosphothreonine. MOD_RES: 168 168, Phosphoserine. MOD_RES: 216 216, Phosphoserine; by CHK1, CHK2 and BRSK1. VAR_SEQ: 66 123, GTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQE VHLAGMNHDQHLMKCSP -> SPGFFRTSGSAFSWD (in isoform 4 and isoform 5). /FTId=VSP_000863. VAR_SEQ: 124 153, Missing (in isoform 2 and isoform 5). /FTId=VSP_000864. VARIANT: 14 14, S -> N (in dbSNP:rs11567959). /FTId=VAR_027922. VARIANT: 70 70, R -> C (in dbSNP:rs3734166). /FTId=VAR_027923. VARIANT: 78 78, S -> N (in dbSNP:rs11567962). /FTId=VAR_027924. VARIANT: 297 297, G -> R (in dbSNP:rs11567997). /FTId=VAR_020146. MUTAGEN: 352 352, E->K: Partial loss of HIV-1 Vpr binding. MUTAGEN: 359 359, K->E: No effect on HIV-1 Vpr binding. STRAND: 127 129,
Key Begin End Length Description RESID
  CHAIN 1 473 473 M-phase inducer phosphatase 3. /FTId=PRO_0000198647.
  DOMAIN 321 428 108 Rhodanese.
  REGION 334 379 46 HIV-1 Vpr binding site.
  ACT_SITE 377 377 1 By similarity. RESID:AA0005; RESID:AA0171; RESID:AA0269
  MOD_RES 38 38 1 Phosphoserine. RESID:AA0037
  MOD_RES 48 48 1 Phosphothreonine. RESID:AA0038
  MOD_RES 57 57 1 Phosphoserine. RESID:AA0037
  MOD_RES 61 61 1 Phosphoserine. RESID:AA0037
  MOD_RES 64 64 1 Phosphoserine. RESID:AA0037
  MOD_RES 67 67 1 Phosphothreonine. RESID:AA0038
  MOD_RES 168 168 1 Phosphoserine. RESID:AA0037
  MOD_RES 216 216 1 Phosphoserine; by CHK1, CHK2 and BRSK1.
  VAR_SEQ 66 123 58 GTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQE VHLAGMNHDQHLMKCSP -> SPGFFRTSGSAFSWD (in isoform 4 and isoform 5). /FTId=VSP_000863.
  VAR_SEQ 124 153 30 Missing (in isoform 2 and isoform 5). /FTId=VSP_000864.
  VARIANT 14 14 1 S -> N (in dbSNP:rs11567959). /FTId=VAR_027922.
  VARIANT 70 70 1 R -> C (in dbSNP:rs3734166). /FTId=VAR_027923.
  VARIANT 78 78 1 S -> N (in dbSNP:rs11567962). /FTId=VAR_027924.
  VARIANT 297 297 1 G -> R (in dbSNP:rs11567997). /FTId=VAR_020146.
  MUTAGEN 352 352 1 E->K: Partial loss of HIV-1 Vpr binding.
  MUTAGEN 359 359 1 K->E: No effect on HIV-1 Vpr binding.
  STRAND 127 129 3


IPI crosslinks:
SP P30307-2 IPI00640320 ENSP00000348898; 1727,CDC25C; 995,CDC25C; UPI000020C6C6 Hs.656;
IPI gene crosslinks:
5 137648858 137695415 -1 5q31 ENSG00000158402 1727,CDC25C 995,CDC25C IPI00216683;IPI00216685;IPI00640320;IPI00216684;IPI00816725; P30307-1;P30307-2;P30307-3;P30307-4; B4DX61;Q9H2F0; ENSP00000321656;ENSP00000345205;ENSP00000348898;ENSP00000349821; REVIEWED:NP_001781;REVIEWED:NP_073720; HIT000022564; Hs.656; CCDS4202.1;CCDS4203.1; GI:12408658;GI:125625350; OTTHUMG00000129203; OTTHUMP00000159489;OTTHUMP00000159490;